Intercalibration of the acridine orange direct count method of aquatic bacteria.
نویسندگان
چکیده
منابع مشابه
Comparison of two direct-count techniques for enumerating aquatic bacteria.
Planktonic bacteria from an estuary were concentrated on membrane filters and counted with both a scanning electron microscope and an epi-illuminated fluorescent microscope. Counts on 0.2 micron Nuclepore filters (polycarbonate) were significantly higher (P less than 0.001) than counts on 0.2-micron Sartorius filters (cellulose). In contrast, there was not a statistically significant difference...
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The direct viable count method first described by Kogure et al. (Can. J. Microbiol. 25:415-420, 1979) was improved by using an antibiotic cocktail instead of nalidixic acid alone. We screened 100 marine isolates from two coastal areas for their sensitivities to five replication-inhibiting antibiotics, including four quinolones (nalidixic, piromidic, and pipemidic acids and ciprofloxacin) and on...
متن کاملDirect acridine orange counting of bacteria preserved with acidified lugol iodine.
Acidified and nonacidified Lugol iodine solution was tested under several storage temperatures and at several times as a preservative for marine bacteria. Direct counts with acridine orange showed no significant difference between glutaraldehyde- and Lugol iodine solution-preserved samples under any storage temperature when samples were counted within 1 week of collection. Specimens in long-ter...
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Dynamics of Acridine Orange-cell Interaction
The in vitro localization of acridine orange (AO) in living cells was monitored by means of fluorescence microscopy, quantitative cell viability studies, and photofluorimetric measurements following dye-cell interaction. The parameters, pH, time, dye concentration, and the metabolic state of the cell were found to exert a profound influence on the time course and distribution of staining. The p...
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ژورنال
عنوان ژورنال: Bulletin of Japanese Society of Microbial Ecology
سال: 1989
ISSN: 0911-7830
DOI: 10.1264/microbes1986.4.89